Stallion Ejaculation Induced by Manual Stimulation of the Penis

From The Journal of Theriogenology, Vol.31 No.2, February 1989
by Jim Crump, Jr. and Julia Crump
Roanoke AI Laboratories, Inc.
8535 Martin Creek Road,
Roanoke, VA 24018

Received for publication: February 25, 1988
Accepted: November 21, 1988


This paper reports the use of a procedure for collection of semen from stallions by manual stimulation of the penis while the stallion is standing. Our use of this method with 18 stallions of various ages and types of semen collection experience indicates that this method may be an efficient alternative to traditional semen collection techniques using an artificial vagina and stimulus mare or dummy mount mare. Our observations, together with those of others who have tried the manual technique, suggest that both animals and handlers can be readily trained to use this method. Limited data suggests that semen samples obtained by manual stimulation are similar to those obtained using an artificial vagina.

Key Words: equine, stallion, semen collection, artificial insemination

The authors wish to thank Drs. R.M. Kenny and Sue M. McDonnell of Hoffman Center for Reproductive Studies, University of Pennsylvania School of Veterinary Medicine, for assistance in preparation of this manuscript.


A number of methods, including condom, vaginal sponge and artificial vagina, have been employed for collection of semen from stallions (1). Currently, the most widely recommended technique is the artificial vagina with a mare or dummy mount. Successful collection of semen using an artificial vagina with the stallion standing on the ground was recently reported (2). Since 1965, we have employed an even simpler method of collecting semen from the stallion using manual stimulation of the penis, and holding a disposable plastic bag over the end of the penis to catch the ejaculate. This technique gradually evolved during our first year of implementing artificial breeding on a small, remote farm, where semen collection equipment was not readily obtainable. Initially, we attempted to fashion a home-made Mississippi style artificial vagina (3) using a latex rubber tube occluded at one end by two sticks and foam rubber padding. While manipulating this flimsy device, we found that manual pressure applied to the penis elicited pelvic thrusting and ejaculation, even before the stallion mounted the mare. Accordingly, we proceeded to collect semen from stallions by manual stimulation and by substituting a plastic bag for the artificial vagina. We continued to use this method and it remains our preferred technique for obtaining semen for our artificial insemination and semen freezing programs.


Plastic bags of various sizes are successfully employed. A bag should extend 8 to 12 in. up the shaft of the penis and be large enough for the glans penis to fully engorge. It is important to use a bag made of material which is not toxic to sperm. We presently use 6" x 10" polyethylene (1.5 mil) bags (Bel-Art Products, Pequannock, NJ). In addition to the bag, we use a 500-ml plastic squeeze bottle filled with warm water (48 to 52 C), which is used to wash the penis and to warm the hand of the operator before manipulation.

The stallions is stimulated to achieve an erection, but is not allowed to mount. Some horses will respond adequately to a mare or gelding presented at a distance, or a brief glimpse of a mare or gelding walking past a doorway; others respond adequately to a dummy mare; still others will respond in their stalls to the operator rubbing the horse's chest, abdomen, flank or thigh. We have also used an in vitro olfactory stimulus (the urine of an estrous mare presented on a paper towel) to arouse a stallion for collection of semen. This stimulus seems to work particularly well with young, inexperienced stallions. We have found that intensely aroused stallions often fail to respond favorably to manipulation. Therefore, our aim is generally to present the least amount of stimulation to achieve and maintain erection.
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When erection is achieved, the penis is rinsed with warm water from the squeeze bottle. This usually elicits some engorgement of the glans penis as well as shallow pelvic thrusting. The plastic bag is then placed over the shaft of the penis and secured with one hand placed proximally to to the preputial ring. The other hand is placed over the glans penis with the thumb behind the corona, as shown in figure 1. [Figure deleted here, unfortunately]. While applying firm pressure, the shaft and glans are gently manipulated in a rhythmic fashion until the horse reponds with pelvic thrusting. As the horse thrusts forward, the hands follow the thrusting motion. Steady pressure is maintained on the shaft. Simultaneously, the glans is rhythmically massaged with rotation motion, with the thumb massaging the firm protuberance of the corpus cavernosum penis into the glans penis. During initial training of a stallion, the position of the hands as well as the rhythm and strength of the pressure applied on the shaft and glans are varied depending on the response of the stallion, with the goal of inducing deep pelvic thrusts and engorgement of the glans characteristic of normal copulatory response. The stallion must be allowed freedom to lower the head when thrusting. Ejaculatory pulses are easily palpated and visualized. Premature release of pressure seems to interrupt ejaculation in some stallions. In fact, a sperm rich portion of the ejaculate may be obtained by releasing manual pressure after one or two urethral pulses.


Table 1 summarizes data on 18 stallions at our facility that were trained to ejaculate in response to manual stimulation. The ejaculates obtained have been used for insemination of mares, as fresh or processed semen (cooled for 24 to 36 h; 4,5).

Table 1.

Summary of data on 18 stallions trained to ejaculate in response to manual stimulation

Animal Age (a) Experience (b) Sessions Required Collection Attempts (c)
Arabian 2 mo none 1 1 to 3
Arabian 3 mo none 2 1 to 3
Arabian 3 mo none 1 1 to 3
Arabian 3 mo none 1 1 to 3
Arabian 5 mo none 1 1 to 3
Arabian 5 mo none 1 1 to 3
Arabian 1.5 yr none 1 1 to 3
Arabian 1.5 yr none 1 1 to 3
Arabian 1.5 yr none 1 1 to 3
Arabian 2 yr natural 1 2 (d)
Arabian 2 yr natural less than 3 1 to 3
Arabian 2 yr none 9 1 to 3
Arabian 3 yr natural 4 1 to 3
Arabian 4 yr none 1 1 to 3
Arabian 4 yr natural 1 1
Arabian 5 yr natural unsuccessful after 22 0
Arabian 12 yr natural less than 3 28
(a) Age at time of training
(b) The number of training sessions before successful ejaculation. Each session consisted of several attempts to collect semen during an approximately 15-min period.
(c) The number of attempts routinely required to collect semen. One attempt consisted of placing the bag on the erect penis and manipulating the penis.
(d) This stallion, over a period of 21 years, consistently would ejaculate during the second attempt.

We have successfully employed this technique with novice and experienced stallions. To better understand developmental changes in semen characteristics and sexual behavior, we have trained several young colts to semen collection procedures and have periodically examined ejaculates throughout the first two years of life. The youngest stallion trained in this collection technique was 2 mo old (Crump, unpublished studies). Other stallions that were trained had previously been bred for many years by natural service or artificial vagina. Training time does not seem to vary systematically with the animal's experience. Generally, training is accomplished within one or two sessions. Novice stallions have produced ejaculates during the first attempt. Training time has varied from one to several attempts for each of 9 sessions. One stallion was never successfully trained after 22 training sessions over a three year period.

The manual stimulation technique was used successfully to collect semen from one Arabian stallion for 21 yr, from 2 to 23 yr of age. The technique does not appear to interfere with natural breeding or other collection techniques, and many stallions with which we worked returned to or alternated between natural service or artificial vagina programs.

In our various uses of semen, we have not detected any systematic differences between ejaculates obtained by this manual method and by traditional artificial vagina methods. For example, Table 2 summarizes nine ejaculates obtained from one stallion over a period of 4 mo using four variations of artificial vagina and manual stimulation methods. As shown, there appear to be no systematic differences between samples obtained by the various collection methods.

Table 2.

Summary of 9 semen samples from a 12 yr Akhel-Teke stallion obtained by three methods.

Method Volume (ml) Semen 
Mar 31 not known AV/ground 20 0 283.0 5.7 75
Mar 31 1 hour AV/mare 65 2 94.0 6.1 75
Apr 16 7 days AV/mare 100 0 124.3 12.4 80
May 23 9 days AV/dummy 82 5 57.5 4.7 90
Jul 06 11 days AV/dummy 65 12 95.0 6.2 85
Jul 07 32 hours AV/dummy 68 4 56.0 3.8 90
Jul 10 2 days AV/dummy 56 5 146.8 8.2 85
Jul 12 2 days Manual 60 0 104.0 6.2 80
Jul 14 2 days Manual 63 5 124.0 7.8 80
(a) (10e6 sperm/ml)
(b) Total number or sperm x 10e9
(c) Visual estimate of percent total motility

Some stallions stop thrusting before ejaculating, or they may achieve engorgement and appear to be near ejaculating, but stop just before. These animals appear to become distracted or over-aroused during collection. In our experience, these problems can often be avoided by providing the minimum stimulus to achieve response. The stallion may, for example, become distracted if he is too near an estrous mare during manipulation. In some instances, an extremely stimulated stallion can be induced to ejaculate simply by applying pressure to only the glans penis.

Most stallions readily become conditioned to this semen collection procedure. They appear to associate breeding with the operator and the plastic bag. Once trained, they often appear more attentive to the operator and the plastic bag than to a mare, much the same as stallions that are trained to an artificial vagina and dummy mare become conditioned to those breeding stimuli. One stallion we worked with routinely achieved erection in the stall when the operator approached with the plastic bag, and with manual stimulation, he ejaculated (without the stimulus of a mare or of an olfactory stimulus). In apparent anticipation of collection, two of our stallions consistently backed away from the stimulus mare toward the operator who was crinkling the plastic bag. Although we routinely use a stimulus mare, stallions experienced with this technique often require less stimulation from the mare than for traditional collection methods.

With minor modifications, this technique has recently been employed with pony stallions at the University of Pennsylvania (McDonnell, personal communication, 1987), where 10 stallions, subjects of a semen study, were readily trained for the collection of semen with a plastic bag and manual stimulation. Five of these 10 stallions consistently responded quickly and ejaculated while standing; the remaining 5 responded with less vigor and were allowed to mount a mare for collection, in which case manual stimulation was performed in place of an artificial vagina. In addition, a warm (45 to 50 C) wet towel compress was added to provide additional stimulation of the glans penis when necessary. All stallions successfully ejaculated within one or two attempts, and continued to be managed in this manner without any complications. Throughout the 3-mo study, semen samples were collected two to three times a week; usually less than 1 h was required to obtain semen samples from the 10 stallions. In this study, the stimulus mare was tethered, so collections were accomplished by one stallion handler and one operator. In similar work (McDonnell, personal communication), collections were made from stallions tethered in their stalls with a stimulus mare tethered nearby. This arrangement permitted one person to conduct the collection. The breeding history of the stallions varied: some had been bred naturally and some had previously been trained to artificial vagina collection of semen. However, all animals readily responded and became conditioned to the manual stimulation method. Subsequently, several student operators have successfully collected semen from these ponies even in their first attempt to employ the technique.

The manual stimulation technique offers several distinct advantages over traditional semen collection methods (artificial vagina on a mount mare). These include 1) markedly reduced collection time and minimal preparation and clean-up time; 2) cleaner samples (plastic bag contacts only lower portion of the shaft of the penis), no lubricating jelly is required; 3) disposable materials, reduced risk of infection; 4) inexpensive materials; 5) heat damage to sperm from contact with artificial vagina reduced; 6) places less physical stress on stallions; 7) allows several ejaculates in quick succession (one stallion produced six ejaculated in less than 20 minutes, another produced three ejaculates in 6 min); 7) it is possible to obtain just the sperm-rich portion of the ejaculate; 8) generally requires fewer personnel; and 9) does not always require a stimulus mare.

While we have preferred to train stallions to ejaculate while standing on the ground, manual stimulation with a plastic bag can be substituted for the artificial vagina with the stallion mounted on a mare or dummy mount.

Potential disadvantages of this technique are that 1) a stallion and operator may require more training than is necessary when an artificial vagina and mount mare technique is used 2) large, tall horses may thrust with enough force to unbalance or knock down the handler, and 3) with tall horses, the operator may be at risk of injury should the horse kick during the procedure. In addition, it is difficult to adequately manipulate the larger glans penis typical or large, tall horses.

We have found collection of semen from stallions by manual stimulation of the penis to be a convenient and efficient technique. It involves our observations over twenty years, together with recent observations of others, indicate that both stallions and operators can be readily trained to use this method.


1. Kenny, R.M., Hurtgen, J.P., Pierson, R., Witherspoon, D. and Simons,J. Clinical fertility evaluation of the stallion. Hastings, NE: Society for Theriogenology (1983)

2. Schumacher, J. and Riddell, M.G. Collection of stallion semen without a mount. Theriogenology 26:245-250 (1986)

3. Berliner, V. Horses and jackstock, In: Perry, E.J. (ed.): The Artificial Insemination of Farm Animals. Rutgers University Press, New Brunswick, NJ, 1960, p.221.

4. Douglas-Hamilton, D.H., Osol, R., Osol, G. and Driscoll D., Noble, H. A field study of the fertility of transported equine semen. Theriogenology 22:291-304 (1984).

5. Cristanelli, M.J., Amann, R.P., Squires, E.L., and Pickett, B.W. Effects of egg yolk and glycerol levels in lactose-EDTA-EGG yolk extender on the motility of frozen-thawed stallion spermatazoa. Theriogenology 24:681-686 (1985).